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human airway smooth muscle (hasm) cells (ScienCell)

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ScienCell human airway smooth muscle (hasm) cells

C o mparing responses <t>in</t> <t>HLF-SC</t> and <t>HHSteC</t> shows both general and selective responses. (a) Scatter plot of average z-scores for contraction for compounds tested in both cell types at the confirmation screen level. Note: not all hits were tested in both cell types, as in this case molecules were only re-tested in the cell type where they were originally identified as hits. (b) Distribution of compounds by affected pathways for confirmed hits in each cell type, highlighting pathway overrepresentation. (c) Dose response curves for selected compounds demonstrating cell-type selectivity among the MYO cells. Compounds labelled with an asterisk (*) were over 10 times more potent (by IC50) in HHSteC.

Human Airway Smooth Muscle (Hasm) Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human airway smooth muscle (hasm) cells - by Bioz Stars, 2026-03

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1) Product Images from "Building an atlas of mechanobiology: high-throughput contractility screen of 2418 kinase inhibitors in five primary human cell types reveals selective divergent responses among related cell types"

Article Title: Building an atlas of mechanobiology: high-throughput contractility screen of 2418 kinase inhibitors in five primary human cell types reveals selective divergent responses among related cell types

Journal: bioRxiv

doi: 10.1101/2025.01.11.632556

C o mparing responses in HLF-SC and HHSteC shows both general and selective responses. (a) Scatter plot of average z-scores for contraction for compounds tested in both cell types at the confirmation screen level. Note: not all hits were tested in both cell types, as in this case molecules were only re-tested in the cell type where they were originally identified as hits. (b) Distribution of compounds by affected pathways for confirmed hits in each cell type, highlighting pathway overrepresentation. (c) Dose response curves for selected compounds demonstrating cell-type selectivity among the MYO cells. Compounds labelled with an asterisk (*) were over 10 times more potent (by IC50) in HHSteC.

Figure Legend Snippet: C o mparing responses in HLF-SC and HHSteC shows both general and selective responses. (a) Scatter plot of average z-scores for contraction for compounds tested in both cell types at the confirmation screen level. Note: not all hits were tested in both cell types, as in this case molecules were only re-tested in the cell type where they were originally identified as hits. (b) Distribution of compounds by affected pathways for confirmed hits in each cell type, highlighting pathway overrepresentation. (c) Dose response curves for selected compounds demonstrating cell-type selectivity among the MYO cells. Compounds labelled with an asterisk (*) were over 10 times more potent (by IC50) in HHSteC.

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Journal: bioRxiv

doi: 10.1101/2025.01.11.632556

Figure Lengend Snippet: C o mparing responses in HLF-SC and HHSteC shows both general and selective responses. (a) Scatter plot of average z-scores for contraction for compounds tested in both cell types at the confirmation screen level. Note: not all hits were tested in both cell types, as in this case molecules were only re-tested in the cell type where they were originally identified as hits. (b) Distribution of compounds by affected pathways for confirmed hits in each cell type, highlighting pathway overrepresentation. (c) Dose response curves for selected compounds demonstrating cell-type selectivity among the MYO cells. Compounds labelled with an asterisk (*) were over 10 times more potent (by IC50) in HHSteC.

Article Snippet: Cryopreserved human primary lung fibroblasts (HLF) and human tracheal), human primary hepatic stellate cells (HHSteC), human airway smooth muscle (HASM) cells, and human bladder smooth muscle (HBSM) cells were obtained from ScienCell.

Techniques:

A: effects of 48-h treatment of the long-chain free fatty acids (oleic acid and linoleic acid) or a selective agonist of FFAR1 (GW9508) on primary cultured human airway smooth muscle (HASM) cells proliferation. Cell proliferation was assayed by the bromodeoxyuridine (BrdU) incorporation assay. Data are shown as percentages of cell proliferation compared with no treatment controls and represent means ± SE. Numbers of experiments are shown in parentheses. B and C: effect of pretreatment of HASM cells with the MEK inhibitor U0126 (5 µM for 2 h) (B) or the phosphoinositide 3-kinase (PI3K) inhibitor LY294002 (3 µM for 60 min) (C) on HASM cells proliferation stimulated by long-chain free fatty acids (oleic acid or linoleic acid; 10 µM each) or a selective agonist of FFAR1 (GW9508; 20 µM) for 48 h. Data are shown as percentages of cell proliferation compared with no treatment controls and represent means ± SE. *P < 0.05; **P < 0.01; ***P < 0.001, compared with no treatment control. #P < 0.05; ##P < 0.01; ###P < 0.001, compared with FFAR1 agonist alone. D: HASM cell viability analysis with MTT assay after 48-h treatment with U0126 (5 µM), LY294002 (3 µM), oleic acid (10 µM), linoleic acid (10 µM), or GW9508 (20 µM); n = 5. Data are shown as percentages of absorbance at 570 nm compared with no treatment control and represent means ± SE. *P < 0.05; ***P < 0.001, compared with no treatment control.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: The free fatty acid receptor 1 promotes airway smooth muscle cell proliferation through MEK/ERK and PI3K/Akt signaling pathways

doi: 10.1152/ajplung.00129.2017

Figure Lengend Snippet: A: effects of 48-h treatment of the long-chain free fatty acids (oleic acid and linoleic acid) or a selective agonist of FFAR1 (GW9508) on primary cultured human airway smooth muscle (HASM) cells proliferation. Cell proliferation was assayed by the bromodeoxyuridine (BrdU) incorporation assay. Data are shown as percentages of cell proliferation compared with no treatment controls and represent means ± SE. Numbers of experiments are shown in parentheses. B and C: effect of pretreatment of HASM cells with the MEK inhibitor U0126 (5 µM for 2 h) (B) or the phosphoinositide 3-kinase (PI3K) inhibitor LY294002 (3 µM for 60 min) (C) on HASM cells proliferation stimulated by long-chain free fatty acids (oleic acid or linoleic acid; 10 µM each) or a selective agonist of FFAR1 (GW9508; 20 µM) for 48 h. Data are shown as percentages of cell proliferation compared with no treatment controls and represent means ± SE. *P < 0.05; **P < 0.01; ***P < 0.001, compared with no treatment control. #P < 0.05; ##P < 0.01; ###P < 0.001, compared with FFAR1 agonist alone. D: HASM cell viability analysis with MTT assay after 48-h treatment with U0126 (5 µM), LY294002 (3 µM), oleic acid (10 µM), linoleic acid (10 µM), or GW9508 (20 µM); n = 5. Data are shown as percentages of absorbance at 570 nm compared with no treatment control and represent means ± SE. *P < 0.05; ***P < 0.001, compared with no treatment control.

Article Snippet: Primary cultured human airway smooth muscle (HASM) cells obtained from Lonza (cc-2576 and 00194850) were derived from three subjects ( subject 1 : 60-yr-old male Caucasian; subject 2 : 56-yr-old male Caucasian; and subject 3 : 27-yr-old male asthmatic Caucasian).

Techniques: Cell Culture, BrdU Incorporation Assay, MTT Assay

Effects of long-chain free fatty acids (oleic acid or linoleic acid) or a selective agonist of FFAR1 (GW9508) on the phosphorylation of ERK and Akt in cultured HASM cells. Cells were stimulated with oleic acid, linoleic acid, or GW9508, and subsequently cell lysates were processed to detect phosphorylated (A, top) and total (A, bottom) levels of ERK or Akt by immunoblot. A: concentration-dependent effect of oleic acid, linoleic acid, and GW9508 (0.5–20 µM; 10 min) on the phosphorylation of ERK (i) and Akt (ii) in cultured HASM cells. B: time-course effect of oleic acid (10 µM), linoleic acid (10 µM), or GW9508 (20 µM) on the phosphorylation of ERK (i) and Akt (ii) in cultured HASM cells. Data are shown as a ratio of phosphorylated to total ERK or Akt and expressed relative to basal (i.e., no treatment control) ratios and are expressed as means ± SE. *P < 0.05; **P < 0.01; ***P < 0.001, compared with basal (time 0). Numbers of experiments are shown in parentheses.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: The free fatty acid receptor 1 promotes airway smooth muscle cell proliferation through MEK/ERK and PI3K/Akt signaling pathways

doi: 10.1152/ajplung.00129.2017

Figure Lengend Snippet: Effects of long-chain free fatty acids (oleic acid or linoleic acid) or a selective agonist of FFAR1 (GW9508) on the phosphorylation of ERK and Akt in cultured HASM cells. Cells were stimulated with oleic acid, linoleic acid, or GW9508, and subsequently cell lysates were processed to detect phosphorylated (A, top) and total (A, bottom) levels of ERK or Akt by immunoblot. A: concentration-dependent effect of oleic acid, linoleic acid, and GW9508 (0.5–20 µM; 10 min) on the phosphorylation of ERK (i) and Akt (ii) in cultured HASM cells. B: time-course effect of oleic acid (10 µM), linoleic acid (10 µM), or GW9508 (20 µM) on the phosphorylation of ERK (i) and Akt (ii) in cultured HASM cells. Data are shown as a ratio of phosphorylated to total ERK or Akt and expressed relative to basal (i.e., no treatment control) ratios and are expressed as means ± SE. *P < 0.05; **P < 0.01; ***P < 0.001, compared with basal (time 0). Numbers of experiments are shown in parentheses.

Techniques: Cell Culture, Western Blot, Concentration Assay

A: representative immunoblot analysis of oleic acid-stimulated phosphorylation of ERK and Akt in primary cultured HASM cells from 3 different donors (subject 1: 60-yr-old male Caucasian; subject 2: 56-yr-old male Caucasian; and subject 3: 27-yr-old male asthmatic Caucasian). Images are representative of at least 3 independent immunoblot analyses. B: effects of oleic acid or GW9508 on the phosphorylation of ERK (i) and (ii) in rat tracheal smooth muscle. Rat tracheal smooth muscle tissue was stimulated with oleic acid (20 µM) or GW9508 (20 µM) for 20 min, and subsequently the tissue homogenates were processed to detect phosphorylated (top) and total (bottom) levels of ERK or Akt by immunoblot. Phosphorylation of ERK or Akt is presented as a ratio of phosphorylated to total ERK or Akt and expressed relative to basal ratios. Data represent means ± SE. *P < 0.05, compared with basal.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: The free fatty acid receptor 1 promotes airway smooth muscle cell proliferation through MEK/ERK and PI3K/Akt signaling pathways

doi: 10.1152/ajplung.00129.2017

Figure Lengend Snippet: A: representative immunoblot analysis of oleic acid-stimulated phosphorylation of ERK and Akt in primary cultured HASM cells from 3 different donors (subject 1: 60-yr-old male Caucasian; subject 2: 56-yr-old male Caucasian; and subject 3: 27-yr-old male asthmatic Caucasian). Images are representative of at least 3 independent immunoblot analyses. B: effects of oleic acid or GW9508 on the phosphorylation of ERK (i) and (ii) in rat tracheal smooth muscle. Rat tracheal smooth muscle tissue was stimulated with oleic acid (20 µM) or GW9508 (20 µM) for 20 min, and subsequently the tissue homogenates were processed to detect phosphorylated (top) and total (bottom) levels of ERK or Akt by immunoblot. Phosphorylation of ERK or Akt is presented as a ratio of phosphorylated to total ERK or Akt and expressed relative to basal ratios. Data represent means ± SE. *P < 0.05, compared with basal.

Techniques: Western Blot, Cell Culture

Effects of oleic acid on phosphorylation of c-Raf in cultured HASM cells. Cells were stimulated with oleic acid, and subsequently cell lysates were processed to detect phosphorylated and total levels of c-Raf by immunoblot. For A–D: i: immunoblot analysis; ii: graphical analysis. A: effects of oleic acid (10 µM) on the phosphorylation of c-Raf at either Ser 338 (n = 6) or Ser 259 (n = 5) in HASM cells. B–D: effects of inhibitors of either Gαi or Gαq protein or Gβγ subunits on oleic acid-induced phosphorylation of c-Raf at Ser 338 in HASM cells. Cells were pretreated with the Gαi-specific inhibitor pertussis toxin (PTX; 100 ng/ml for 4 h; n = 5; B), Gαq-specific inhibitor YM-254890 (1 µM for 30 min; n = 8; C), or Gβγ signaling inhibitor gallein (10 µM for 30 min; n = 7; D) before treatment with oleic acid (10 µM) for 10 min. Phosphorylation of c-Raf is presented as a ratio of phosphorylated to total c-Raf and then normalized to basal levels. Data represent means ± SE. *P < 0.05; ***P < 0.001, compared with basal.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: The free fatty acid receptor 1 promotes airway smooth muscle cell proliferation through MEK/ERK and PI3K/Akt signaling pathways

doi: 10.1152/ajplung.00129.2017

Figure Lengend Snippet: Effects of oleic acid on phosphorylation of c-Raf in cultured HASM cells. Cells were stimulated with oleic acid, and subsequently cell lysates were processed to detect phosphorylated and total levels of c-Raf by immunoblot. For A–D: i: immunoblot analysis; ii: graphical analysis. A: effects of oleic acid (10 µM) on the phosphorylation of c-Raf at either Ser 338 (n = 6) or Ser 259 (n = 5) in HASM cells. B–D: effects of inhibitors of either Gαi or Gαq protein or Gβγ subunits on oleic acid-induced phosphorylation of c-Raf at Ser 338 in HASM cells. Cells were pretreated with the Gαi-specific inhibitor pertussis toxin (PTX; 100 ng/ml for 4 h; n = 5; B), Gαq-specific inhibitor YM-254890 (1 µM for 30 min; n = 8; C), or Gβγ signaling inhibitor gallein (10 µM for 30 min; n = 7; D) before treatment with oleic acid (10 µM) for 10 min. Phosphorylation of c-Raf is presented as a ratio of phosphorylated to total c-Raf and then normalized to basal levels. Data represent means ± SE. *P < 0.05; ***P < 0.001, compared with basal.

Techniques: Cell Culture, Western Blot

Effects of oleic acid, linoleic acid, or GW9508 on phosphorylation of p70S6K and S6 ribosomal protein in cultured HASM cells. Cells were stimulated with oleic acid, linoleic acid, or GW9508, and subsequently cell lysates were processed to detect phosphorylated and total levels of p70S6K or S6 ribosomal protein by immunoblot. For A–E: i: immunoblot analysis; ii: graphical analysis A: concentration-dependent effect of oleic acid, linoleic acid, and GW9508 (0.5–0 µM; 10 min) on the p70S6K phosphorylation in cultured HASM cells. B: time-course effect of oleic acid (10 µM), linoleic acid (10 µM), and GW9508 (20 µM) on the p70S6K phosphorylation in cultured HASM cells. C: effect of the PI3K inhibitor LY294002 (3 µM for 60 min) or the MEK inhibitor U0126 (5 µM for 2 h) on oleic acid (10 µM; 10 min)- or GW9508 (20 µM; 10 min)-induced p70S6K phosphorylation in HASM cells. D: effect of the mammalian target of rapamycin (mTOR) inhibitor rapamycin (1 µM for 60 min) on oleic acid- or GW9508-induced phosphorylation of p70S6K in HASM cells. E: effect of rapamycin (1 µM for 60 min) on oleic acid- or GW9508-induced phosphorylation of S6 ribosomal protein in HASM cells. Phosphorylation of p70S6K and S6 ribosomal protein are presented as a ratio of phosphorylated to total p70S6K or S6 and expressed relative to basal ratios. Data are shown as percentages of basal cell phosphorylation and are expressed as means ± SE. *P < 0.05; **P < 0.01; ***P < 0.001, compared with basal (time 0). Numbers of experiments are shown in parentheses.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: The free fatty acid receptor 1 promotes airway smooth muscle cell proliferation through MEK/ERK and PI3K/Akt signaling pathways

doi: 10.1152/ajplung.00129.2017

Figure Lengend Snippet: Effects of oleic acid, linoleic acid, or GW9508 on phosphorylation of p70S6K and S6 ribosomal protein in cultured HASM cells. Cells were stimulated with oleic acid, linoleic acid, or GW9508, and subsequently cell lysates were processed to detect phosphorylated and total levels of p70S6K or S6 ribosomal protein by immunoblot. For A–E: i: immunoblot analysis; ii: graphical analysis A: concentration-dependent effect of oleic acid, linoleic acid, and GW9508 (0.5–0 µM; 10 min) on the p70S6K phosphorylation in cultured HASM cells. B: time-course effect of oleic acid (10 µM), linoleic acid (10 µM), and GW9508 (20 µM) on the p70S6K phosphorylation in cultured HASM cells. C: effect of the PI3K inhibitor LY294002 (3 µM for 60 min) or the MEK inhibitor U0126 (5 µM for 2 h) on oleic acid (10 µM; 10 min)- or GW9508 (20 µM; 10 min)-induced p70S6K phosphorylation in HASM cells. D: effect of the mammalian target of rapamycin (mTOR) inhibitor rapamycin (1 µM for 60 min) on oleic acid- or GW9508-induced phosphorylation of p70S6K in HASM cells. E: effect of rapamycin (1 µM for 60 min) on oleic acid- or GW9508-induced phosphorylation of S6 ribosomal protein in HASM cells. Phosphorylation of p70S6K and S6 ribosomal protein are presented as a ratio of phosphorylated to total p70S6K or S6 and expressed relative to basal ratios. Data are shown as percentages of basal cell phosphorylation and are expressed as means ± SE. *P < 0.05; **P < 0.01; ***P < 0.001, compared with basal (time 0). Numbers of experiments are shown in parentheses.

Techniques: Cell Culture, Western Blot, Concentration Assay

Representative immunoblot analyses using antibodies against the dopamine D 1 receptor using total protein prepared from freshly dissected native human tracheal epithelium (20 μg), primary cultured human airway epithelial cells (100 μg), the human bronchial epithelial cell line (16HBE14o-) (100 μg), the human pulmonary mucoepidermoid carcinoma cell line (NCI-H292) (100 μg), and human airway smooth muscle cells (positive control) (100 μg). Reprobing of blots for GAPDH was performed to demonstrate relative lane loading. Each image is representative of at least 3 independent immunoblots

Journal: Respiratory Research

Article Title: The dopamine D 1 receptor is expressed and induces CREB phosphorylation and MUC5AC expression in human airway epithelium

doi: 10.1186/s12931-018-0757-4

Figure Lengend Snippet: Representative immunoblot analyses using antibodies against the dopamine D 1 receptor using total protein prepared from freshly dissected native human tracheal epithelium (20 μg), primary cultured human airway epithelial cells (100 μg), the human bronchial epithelial cell line (16HBE14o-) (100 μg), the human pulmonary mucoepidermoid carcinoma cell line (NCI-H292) (100 μg), and human airway smooth muscle cells (positive control) (100 μg). Reprobing of blots for GAPDH was performed to demonstrate relative lane loading. Each image is representative of at least 3 independent immunoblots

Article Snippet: Primary cultured human airway smooth muscle cells (HASM; cc-2576, Lonza) were grown in DMEM/F12 culture medium, supplemented with 10% FBS and an antibiotic-antimycotic mix (100 units/ml penicillin G sodium, 100 μg/ml streptomycin sulfate, 0.25 μg/ml amphotericin B).

Techniques: Western Blot, Cell Culture, Positive Control

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1) Product Images from "Building an atlas of mechanobiology: high-throughput contractility screen of 2418 kinase inhibitors in five primary human cell types reveals selective divergent responses among related cell types"

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